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. 2004 Jun 28;101(27):10084–10089. doi: 10.1073/pnas.0402930101

Fig. 3.

Fig. 3.

Analysis of lipid in the milk of Btn1a1+/+, Btn1a1+/-, and Btn1a1-/- animals. (A) Amount of milk lipid (%, wt/vol) in the first and first half of the second lactations of BtnKO1 and BtnKO2 strains, as indicated (values, mean ± SD, n = 5 animals per genotype). Btn1a1+/+ (filled squares), Btn1a1+/- (open squares), and Btn1a1-/- (filled triangles) are shown. (B) Phase contrast micrographs of lipid droplets from milk of the three genotypes of BtnKO2 strain mice, as indicated. (Bars: 25 μm.) (C) Profiles of lipid droplet size in milk from the three genotypes on day 10 of the first and second lactations of BtnKO1 and BtnKO2 lines as indicated (average of five lipid samples per genotype). Btn1a1+/+ (solid line), Btn1a1+/- (dashed line), and Btn1a1-/- (dotted line) are shown. (Insets) Mean diameter of lipid droplets from the same samples: Btn1a1+/+ (black bars), Btn1a1+/- (gray bars), and Btn1a1-/- (white bars) are shown (values are mean ± SD, n = 5 samples per genotype). *, Btn1a1-/- values significantly different from Btn1a1+/+ and Btn1a1+/- mice (P < 0.01).