Fig E4.

SOCS1 overexpression increased RV1B-, IL-1β– and TNF-α–induced CXCL8 promoter activation. BEAS-2B cells were seeded and transfected with SOCS1 or the empty vector control pORF and infected with RV1B or treated with 10 ng/mL IL-1β or TNF-α. SOCS1 overexpression increased RV1B-induced (A), IL-1β–induced (B), and TNF-α–induced (C) CXCL8 promoter activation at 24 hours. Values are presented as means ± SEMs (n = 4 experiments). *P < .05 and ***P < .001, 1-way ANOVA with the Bonferroni multiple comparison test. ns, Not significant.