Table E3.
TaqMan real-time RT-PCR primers and probes used for quantitative PCR
| Name | Sequence (5′-3′) |
|---|---|
| hIFN-β forward (300 nmol/L) | cgccgcattgaccatcta |
| hIFN-β reverse (900 nmol/L) | ttagccaggaggttctcaacaatagtgtca |
| hIFN-β probe (FAM 5′-TAMRA 3′ [100 nmol/L]) | tcagacaacattcatctagcactggctgga |
| hIFN-λ1/IL-29 forward (300 nmol/L) | ggacgccttggaagagtcact |
| hIFN-λ1/IL-29 reverse (900 nmol/L) | agaagcctcaggtcccaattc |
| hIFN-λ1/IL-29 probe (FAM 5′-TAMRA 3′ [100 nmol/L]) | agttgcagctctcctgtcttccccg |
| hIFN-λ2/3/IL-28AB forward (300 nmol/L) | ctgccacatagcccagttca |
| hIFN-λ2/3/IL-28AB reverse (900 nmol/L) | agaagcgactcttctaaggcatctt |
| hIFN-λ2/3 probe (FAM 5′-TAMRA 3′ [100 nmol/L]) | tctccacaggagctgcaggccttta |
| hSOCS1 forward (900 nmol/L) | ccctggttgttgtagcagctt |
| hSOCS1 reverse (900 nmol/L) | ggtttgtgcaaagatactgggtatat |
| hSOCS1 probe (FAM 5′-TAMRA 3′ [100 nmol/L]) | aggtaggaggtgcgagttcaggtcctg |
| hSOCS3 forward (900 nmol/L) | tgggacgatagcaaccacaa |
| hSOCS3 reverse (900 nmol/L) | cgaagtgtcccctgtttgga |
| hSOCS3 probe (FAM 5′-TAMRA 3′ [100 nmol/L]) | tggattctccttcaattcctcagcttccc |
| mSOCS1 forward (900 nmol/L) | ccgtgggtcgcgagaac |
| mSOCS1 reverse (900 nmol/L) | aaggaactcaggtagtcacggagta |
| mSOCS1 probe (FAM 5′-TAMRA 3′ [100 nmol/L]) | tggcgcgcatccctcttaaccc |
| mIFN-β forward (900 nmol/L) | ctccacgctgcgttcctgctgtg |
| mIFN-β reverse (900 nmol/L) | aaaggattgccacattgctc |
| mIFN-β probe (FAM 5′-TAMRA 3′ [100 nmol/L]) | tcaagcagcctcttctcgat |
| mTNF-α forward (900M) | agcctgtagcccacgtcgta |
| mTNF-α reverse (900M) | ttgagatccatgccgttgg |
| mTNF-α probe (FAM 5′-TAMRA 3′ [100 nmol/L]) | aaccaccaagtggaggagcagctgg |
| 18S forward (300 nmol/L) | cgccgctagaggtgaaattct |
| 18S reverse (300 nmol/L) | cattcttggcaaatgctttcg |
| 18S probe (FAM 5′-TAMRA 3′ [100 nmol/L]) | accggcgcaagacggaccaga |
h, Human; m, mouse.