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. 2004 Jun 24;101(27):10116–10121. doi: 10.1073/pnas.0403744101

Fig. 4.

Fig. 4.

TLR stimulation up-regulates cytotoxicity and cytokine release by human NK clones. (a) Two representative NK clones, characterized by the NKG2A+KIR- (50D) or NKG2A-KIR+ (10D) phenotype, were cultured in medium supplemented with IL-2 (10 units/ml) or IL-12 (1 ng/ml) in either the absence (black boxes) or presence of ODN A (black circles), ODN B (black triangles), or poly(I·C) (×) for 20 h and were then assessed for cytotoxicity against the FO-1 target cell line at different E/T ratios. Each value represents the mean of triplicate experiments. The SD did not exceed 4% in the cytotoxicity assays. (b) The same NK clones were cultured with medium (black boxes), ODN A (white boxes), ODN B (dark-gray boxes), or poly(I·C) (light-gray boxes) in the presence of IL-2 (10 units/ml) or IL-12 (1 ng/ml). After 20 h of culture, supernatants were harvested and assessed for IFN-γ, TNF-α, and GM-CSF content by specific ELISA (n = 3, mean ± SD). These data are representative of at least five different experiments.