Fig. 5.

Regulation by CD4⁺CD25⁺ T cells from chimeric mice shows no specificity for the tolerated antigen. H2-D^b-CD4⁺CD25⁺ and H2-D^b-CD4⁺CD25^- T cells were sorted from the spleen of CBA tolerized 100 days before to B10 skin with donor BM and mAb treatment as described. CD4⁺ T cells were isolated from the spleen of naïve CBA mice. RAG1^-/- mice were injected with 10⁵ CD4⁺ T cells alone (•, n = 10; ○, n = 5), or together with 2 × 10⁵ H2-D^b-CD4⁺CD25⁺ (▪, n = 9; □ n = 4) or H2-D^b-CD4⁺CD25^- (▵, n = 5) T cells. In two groups of mice, 2 × 10⁵ H2-D^b-CD4⁺CD25^- T cells were injected alone (▾, n = 10; ▿, n = 4). The transfused mice were transplanted with B10 (open symbols) or BALB/c (filled symbols) skin grafts. P values for the comparisons were as follows: ▾ vs. ▿, P < 0.002; □ vs. ○, P < 0.015; ▪ vs. •, P < 0.003.