Fig. 2.

Aggresomal markers in the CryAB^R120G aggregates. The CryAB^R120G-induced aggregates are immunoreactive with antibodies for ubiquitin, HSP25, the endoplasmic reticulum complex marker protein SEC61α, and tubulin. For ubiquitin, HSP25, and SEC61α, actin is stained red (phalloidin). For tubulin, actin is stained green and tubulin is red. Nuclei are stained blue with To-Pro-3. The bottom row shows a ×2 magnification of the perinuclear regions of the row above. Arrowheads indicate immunopositive aggregates. Veh., empty adenovirus vector.