Effect of ORM1 or ORM1-monocytes on the resistance of the chimeras infected with MRSA or E. faecalis. A. MRSA infection. The chimeras were injected with ORM1-monocytes (i.v., 5 × 106 cells/chimera), ORM1 (i.v., 6 mg/chimera), untreated monocytes (i.v., 5 × 106 cells/chimera, positive control) or saline (0.2 ml/chimera, negative control). Then, all of chimeras were i.v. infected with 2 × 105 CFU/chimera of MRSA. Three days after infection, the growth of bacteria in the liver, spleen and kidneys of these chimeras were determined by a colony-counting method. B.
E. faecalis infection. After antibiotic decontamination and proton-pump inhibitor treatment, four groups of the chimeras shown in A were orally infected with 3 × 105 CFU/chimera of E. faecalis. Three days after infection, the growth of bacteria in the liver and mesenteric lymph nodes (MLNs) of these chimeras were determined by a colony-counting method. Data are displayed by the mean ± SEM from 3 or 4 chimeras per group. Data are representative of at least two independent experiments. * P < 0.05, ** P < 0.01.