Fig. 2.

Strain-specific and crossreactive nAb responses in patient H. (A) Sequential plasma samples from chronically infected patient H were monitored for their ability to neutralize pseudotype viruses bearing H77, H, and HCJ4 gps at final dilution of 1/200. Data are shown as percentage neutralization. (B) Neutralization titer of plasma for HIV-HCV H77, defined as the dilution of plasma able to reduce virus infectivity by 90%. (C) Plasma samples (tested at a dilution of 1/100) were tested for anti-NS3 (circle) and anti-E1E2 (square) IgM (open symbols) and IgG (filled symbols) responses. Data are represented as a P/N ratio, calculated by dividing the OD value of a test serum by that obtained with an irrelevant HCV-negative human serum. P/N values >2 were considered positive. All infections were performed in quadruplicate, and the data are representative of two independent experiments.