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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Heart Rhythm. 2015 Feb 19;12(6):1306–1314. doi: 10.1016/j.hrthm.2015.02.019

Figure 3. Kcnh2 transcripts follow a circadian pattern of expression in rat hearts and the human Kcnh2 promoter is transactivated by the co-expression of BMAL1 and CLOCK.

Figure 3

A) The circadian expression profile for Kcnh2 in the WKY rat heart is shown (n=4 animals per time point). Shaded and light regions represent subjective dark and light cycle. B) BMAL1 and CLOCK transactivate the human Kcnh2 promoter reporter. Luciferase assay results of transfection experiments using the hKcnh2 reporter gene in C2C12 myoblasts (n = 6–10/condition). CLOCK (C) and BMAL1 (B) significantly transactivate the hKcnh2 reporter gene (solid bars) relative to control transfections (open bars). Activation of the hKcnh2 reporter is significantly diminished when Bmal1R91A was overexpressed with CLOCK (gray bar) or ClockΔ19 was overexpressed with BMAL1 (vertical striped bar). Values are expressed as means ± SE. *P < 0.05 compared with the hKcnh2 reporter gene; #P < 0.05 compared with hKcnh2 reporter gene + CLOCK + BMAL.