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. 2004 Jun 21;101(27):10177–10182. doi: 10.1073/pnas.0401229101

Fig. 1.

Fig. 1.

Progenitor cells in the adult rodent SN do not display neuronal or dopaminergic phenotypes. (a) Distribution of BrdUrd (red) and TH (green) immunoreactive cells in the mouse SN after 21 days of BrdUrd (75 mg/ml) minipump infusion into the right lateral ventricle. (b) Laser confocal (Leica) image of a BrdUrd-labeled cell adjacent to a TH-labeled cell, viewed in three dimensions. Note that this is not a double-labeled cell because the orthogonal view reveals that the BrdUrd-labeled nucleus is not completely encapsulated by the TH-labeled cytoplasm. (c) Distribution of BrdUrd (red) and TH (green) immunoreactive cells in the 6-OHDA-lesioned rat SN after BDNF infusion (20). Note the dramatic reduction of TH-labeled cells in the SN as compared with the nonlesioned condition in a. (d) Confocal image of a BrdUrd-labeled cell adjacent to a TH-labeled cell from c. No BrdUrd+ cells coexpressed TH. (e) Confocal image of a red BrdUrd-labeled cell adjacent to a blue TH-labeled cell with its own green NeuN-labeled nucleus, demonstrating that the BrdUrd nucleus did not originate from the TH neuron. (Scale bar = 100 μmin a and c and 5 μm in b, d, and e.)