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Nanohole substrate for multiplexed biosensing and lens-free imaging. (a) SEM images of 6 pixels of nanohole arrays. Each pixel is 100 µm × 100 µm with hole size 200 nm; (b) Schematic of nanohole arrays coated with different proteins: Monolayer of BSA (M), bilayer of protein and IgG (B); (c) Transmission of bare nanohole arrays (red), with protein monolayer (green) and bilayer (blue); (d) diffraction patterns of nanohole arrays before and after functionalization with protein [156].
