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Effect of Ca2+ concentrations on the efficiency of D-loop formation. The human and yeast Rad51-ssDNA filaments (filled and open circles, respectively) were formed by incubation of the protein with 32P-labeled ssDNA (90-mer, no. 90) in the presence of indicated Ca2+ concentrations. JM formation was initiated by addition of pUC19 dsDNA and carried out for 10 min.
