Table 3. The six most abundant bacterial and archaeal operational taxonomic units (OTUs) in enrichment cultures, determined by performing PCR amplification, sequencing and phylogenetic analysis of 16S rRNA genes.
| Phylum | Taxonomic affiliationa |
Percentage of reads in each taxon |
||
|---|---|---|---|---|
| NAPDC | SCADC | TOLDC | ||
| Bacterial OTUs | ||||
| Firmicutes | Anaerobacter | 9.5 | 3.0 | 30.2 |
| Firmicutes | Lachnospiraceae | 0.0 | 0.0 | 23.8 |
| Firmicutes | Peptococcaceae | 19.5 | 0.2 | 0.0 |
| Firmicutes | Desulfotomaculum | 15.3 | 0.0 | 0.0 |
| Firmicutes | Sedimentibacter | 0.0 | 0.0 | 11.1 |
| Firmicutes | Desulfosporosinus | 4.0 | 0.0 | 3.0 |
| Spirochaetes | Spirochaetaceae | 0.6 | 6.5 | 2.2 |
| Spirochaetes | Spirochaetaceae | 2.7 | 14.3 | 0.2 |
| Deltaproteobacteria | Smithella | 0.6 | 11.6 | 0.0 |
| Deltaproteobacteria | Syntrophus | 0.1 | 6.8 | 0.0 |
| Deltaproteobacteria | Geobacter | 2.8 | 2.6 | 0.1 |
| Deltaproteobacteria | Desulfovibrio | 0.6 | 4.2 | 0.0 |
| Deltaproteobacteria | Desulfobacterium | 0.1 | 3.6 | 0.0 |
| Synergistetes | Thermanaerovibrio | 4.2 | 2.5 | 0.0 |
| Chloroflexi | Anaerolineaceae | 0.0 | 0.0 | 5.4 |
| Candidate division | Candidate division OP11 | 2.4 | 0.0 | 0.0 |
| Candidate division | Candidate division OP8 | 0.0 | 0.0 | 3.3 |
| Archaeal OTUs | ||||
| Euryarchaeota | Methanosaeta | 51.5 | 55.9 | 50.6 |
| Euryarchaeota | Methanosaeta | 5.4 | 5.4 | 2.2 |
| Euryarchaeota | Methanoculleus | 32.1 | 21.3 | 15.1 |
| Euryarchaeota | Methanoculleus | 3.6 | 2.4 | 0.4 |
| Euryarchaeota | Methanolinea | 0.4 | 1.3 | 27.3 |
| Euryarchaeota | Candidatus Methanoregula | 0.6 | 4.8 | 0.6 |
| Euryarchaeota | Methanomicrobia | 1.4 | 1.4 | 0.0 |
| Euryarchaeota | WCHA1 | 0.2 | 0.3 | 3.0 |
Abbreviations: NAPDC, naphtha-degrading culture; SCADC, short chain alkane-degrading culture; TOLDC, toluene-degrading culture.
a
Each OTU was assigned a taxonomic affiliation using RDP classifier. Only the lowest identified taxon is shown.