Table 1. The relative abundance of the nitrite reductase gene and transcript responsible for N2O emission in a soil microcosm.
Treatmentsa | nirK in Cluster Ib | nirK in Cluster II | nirK in Cluster III | nirK in Cluster IV | Total nirK | nirS in Cluster I | nirS in Cluster II | Total nirS | Total Nitrite reductase gene | |
---|---|---|---|---|---|---|---|---|---|---|
Gene | NF-8 | 0.506 | 0.717 | 2.191 | 0.027 | 3.441 | 1.863 | 0.498 | 2.36 | 5.802 |
OF-8 | 5.631 | 5.272 | 1.282 | 0.014 | 12.199 | 1.344 | 0.275 | 1.619 | 13.818 | |
Transcript | NF-8 | 0.002 | 0.003 | 0.001 | —c | 0.006 | — | — | — | 0.006 |
OF-8 | 0.010 | 0.049 | 0.002 | — | 0.062 | 0.001 | — | 0.001 | 0.063 |
NF-8 and OF-8 represent the control and organic fertilized soil on the eighth day of N2O flux in the soil microcosm, which corresponds to the curve in Supplementary Figure S3.
Relative abundance of the nitrite reductase gene or transcript copies calculated as a percentage of the total bacterial 16S rRNA gene copies (for the mean and s.d. of replicates by treatment see Supplementary Table S4).
Not detected.