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. Author manuscript; available in PMC: 2016 Jul 2.
Published in final edited form as: Cell Stem Cell. 2015 Jun 18;17(1):35–46. doi: 10.1016/j.stem.2015.05.003

Figure 5. Hierarchical organization and molecular priming.

Figure 5

(A) Differentiation in vitro. Representative FACS plots showing HSCLT and HSCST differentiation kinetics in myeloid conditions (n = 2).

(B) Size of the indicated BM populations in Mpl−/− and littermate control mice (3 mice/group).

(C) Differentiation in vivo. Representative FACS plots of LSK and myeloid progenitor output 10 days following transplantation of 5,000 cells of the indicated donor population (3 mice/group).

(D) Fluidigm gene expression analyses at steady state. Results are expressed as mean (bar) and individual fold differences compared to HSCLT (8-12 pools of 100 cells /population; nd: not detectable).

(E) t-distributed stochastic neighbor embedding (tSNE) analysis of Fluidigm gene expression data acquired from single cells (30-58 cells/population).

Results are expressed as mean ± SD; op < 0.05, •p < 0.01, *p < 0.001.

See also Figures S4 and S5.