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. Author manuscript; available in PMC: 2016 Jul 2.
Published in final edited form as: Cell Stem Cell. 2015 Jun 18;17(1):35–46. doi: 10.1016/j.stem.2015.05.003

Figure 6. Contribution to blood regeneration.

Figure 6

(A) Experimental scheme for in vivo blood regeneration experiments. Donor HSCLT were transplanted into sub-lethally irradiated congenic recipients (2,000 HSCLT/mouse) and regeneration of donor BM subsets was followed at 2 and 3 weeks post-transplantation and compared to steady state control (Ctrl) mice.

(B) Representative FACS plots of regenerating BM subsets.

(C) Frequency of the indicated BM subsets (6-10 mice/group).

(D) Methylcellulose clonogenic assays for the indicated populations (n = 1-4)

(E) PC analysis of Fluidigm gene expression data from the indicated populations (8-12 pools of 100 cells/condition).

Results are expressed as mean ± SD or SEM (D); op < 0.05, •p < 0.01, *p < 0.001.

See also Figure S5, S6.