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CodY binds directly to three sites in the fla/che operon. Results of electrophoretic mobility gel shift analysis of DNA fragments (5 nM) centered on the putative CodY binding motif incubated with the indicated concentrations of 6His-CodY are shown. Gel shifts were performed in the presence of the CodY cofactors GTP, isoleucine, leucine, and valine.
