FIG 1.
Schematic representation of the C-terminal HybC constructs. (A) Schematic representation of N-terminally epitope-tagged HybC derivatives. Pro-HybC represents the unprocessed large subunit of HybC; HybCproc is the genetically truncated version lacking 15 amino acids at its C terminus and is equivalent to processed HybC present in the active Hyd-2 enzyme. Derivatives carrying an N-terminal Strep tag were prepared (not represented in the figure). (B) Schematic representation of the constructs in which the 15-amino-acid C terminus from HyaB was swapped onto HybC and, vice versa, the 15-amino-acid C-terminal peptide from HybC was introduced onto HyaB.