TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Genotype	Reference
Strains
MC4100	F− araD139 α(argF-lac)U169 ptsF25 deoC1 relA1 flbB5301 rspL150	57
DHPF2	MC4100 ΔhypF aaa 59–629	34
DHP-D	MC4100 ΔhypD	58
DHP-E	MC4100 ΔhypE	58
DHP-B	MC4100 ΔhypB	58
DHB-G	MC4100 ΔhybG	30
FTD147	ΔhyaB ΔhybC ΔhycE	45
FTD150	ΔhyaB ΔhybC ΔhycE ΔhyfB-R	45
CP793	MC4100 ΔhyaB ΔhycE ΔhybO::Kanr	39
CP695	MC4100 ΔhybC	39
CB10	DHPF2 ΔhybC	This study
CB11	MC4100 ΔhybG::Kanr	This study
CB13	DADE ΔhybC::Kanr	This study
CB15	FTD147 hybC+ ΔhybG::Kanr	This study
CB16	FTD150 hyaB+ ΔhyaD::Kanr	This study
DADE	MC4100 ΔtatA-E	59
Plasmids
pASK-hyaB	hyaB in pASK-IBA5+, Ampr	39
pASK-hybC	hybC in pASK-IBA5+, Ampr	39
pASK-hybCproc	pASK-hybC, V553 stop (codon 553 converted to TAA), Ampr	This study
pASK-hyaBhybC	hyaB with hybC C-terminal sequence in pASK-IBA7, Ampr	This study
pASK-hybChyaB	hybC with hyaB C-terminal sequence in pASK-IBA7, Ampr	This study

^aaa, amino acids.