FIG 2.

Temporal expression of iron-regulated genes and two major ETEC virulence genes under iron starvation. Gene expression analysis of ETEC H10407 was performed following growth to exponential phase (2 h) or early stationary phase (5 h) in CFA medium alone or supplemented with 50 μM deferoxamine. Data are represented as the fold change ratio of expression in treated versus untreated cultures, calculated from relative expression values using the 2^−ΔΔCT method. Values are log₂ transformed. Means from at least three independent replicates are shown, with error bars representing the SEM. Statistical analyses were performed using the one-sample t test. *, P ≤ 0.05.