FIG 3 .

Properly folded FHA is necessary and sufficient for adherence of B. bronchiseptica to the respiratory tract. (a) CFU recovered in BALF 30 to 60 min after intranasal inoculation of mice. Each point represents the number of CFU recovered from a single animal. (b) Recovery of bacteria in BALF represented as the percentage of the inoculum for each strain. Only strains lacking a properly folded MCD displayed decreased retention in the murine respiratory tract. The FhaB molecules translated by each strain are diagrammed at the top of the figure (dark blue, signal peptide; pink, TPS domain; purple, β-helical domain; green, MCD; brown, PNT; light blue, uncharacterized prodomain; red, PRR; yellow, ECT). Data are pooled from 2 separate experiments conducted on different days. Percentages of inoculum data are represented as means ± standard errors of the means (SEM). ***, P < 0.001.