TABLE 2.
Kinetic constants of the wild-type (WT) and mutant (N174Y) d-lactate dehydrogenase DLDH744 for NADH and NADPH
| Enzyme | Means ± SD of kinetic constants for cofactor: |
|||||||
|---|---|---|---|---|---|---|---|---|
| NADH |
NADPH |
|||||||
| Km (mM) | Vmax (U/mg) | kcat (min−1) | kcat/Km (mM−1 s−1) | Km (mM) | Vmax (U/mg) | kcat (min−1) | kcat/Km (mM−1 s−1) | |
| WT | 0.114 ± 0.0056 | 7.50 ± 0.168 | 525.20 ± 11.80 | 77.02 ± 1.73 | 0.036 ± 0.0004 | 4.66 ± 0.014 | 326.53 ± 0.98 | 149.31 ± 0.45 |
| N174Y | 0.080 ± 0.0003 | 6.93 ± 0.001 | 485.28 ± 0.14 | 100.72 ± 0.03 | 0.056 ± 0.0002 | 0.91 ± 0.001 | 63.56 ± 0.04 | 18.77 ± 0.01 |