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Primers and probe used in the qPCR
| Primer or probe | Sequence (5′–3′)a | Locationb | Amplicon size (bp) |
|---|---|---|---|
| QKF3 | GTGGTGCCAAGCGATTTTAT | 7216–7235 | 78 |
| QKR3 | GTTTCATCCGCGGTGTTAAT | 7293–7274 | |
| QKP3 | FAM-TTTAGCGAGCGAAGCGGTGG-TAMRA | 7253–7272 |
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.
Positions in the whole-genome sequence of C. burnetii RSA493 (GenBank accession number AE016828) that encode the transposase gene of the C. burnetii-specific IS1111a insertion element.
