TABLE 2.
Primers for 16S rRNA gene and gyrB multiallele typing
| Cardinium target | Primer | Sequence (5′→3′) | Amplicon size (bp) | Reference |
|---|---|---|---|---|
| 16S rRNA gene amplicon 1 | 16SA1 F | AGCGGGACACTTCGGTGTTG | 745 | This study |
| 16SA1 R | TCATCGTTTACGGCGTGGAC | |||
| 16S rRNA gene amplicon 2 | 16SA2 F | CGTAGGCGGCTTATTAAGTC | 900 | This study |
| 16SA2 R | GTCCCAGTCGCTGGTCTAAC | |||
| gyrB amplicon 1 | gyrBA1 F | CATGGCGTGGGTATTTCTT | 724 | This study |
| gyrBA1 R | CAGGTTTCTACCGCTCCTTG | |||
| gyrB amplicon 2 | gyrBA2 F | TGCGGATAAATCTGGTCTGC | 841 | This study |
| gyrBA2 R | GCTGTACATACACGGCATCAAC |