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Iron sequestration and catalase abolish hydroxyl-radical formation. Hydroxyl radicals formed in BHI broth medium were detected by ESR (40 mM PBN and 2% [vol/vol] ethanol) in the presence of iron chelator 2,2′-bipyridyl (5 mM [filled triangles] or 50 mM [open circles]) or Chelex 100 (filled squares) or under untreated conditions (filled diamonds) (A) or after addition of catalase at 3 h (1 U/ml) (filled upside-down triangles) compared to the untreated control (filled circles) (B).
