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Low-temperature activities of the WT and its variants. The azocaseinolytic activities of the enzymes (0.75 μg/ml) were determined at 25°C. The values are means ± standard deviations (bars) of three independent experiments. The specific activities of the variants relative to the WT were calculated. Filled circles indicate the variants with VRs that are identical in S41 and Sph. The dashed lines mark the activity levels of the WT.
