TABLE 1.
Substrate | Specific activity (U/μmol)a |
|||
---|---|---|---|---|
WT protease at: |
PBL5X at: |
|||
25°C | 90°C | 25°C | 90°C | |
Casein | 3.0 ± 0.2 | 48.1 ± 3.3 | 12.5 ± 0.5 | 81.9 ± 5.6 |
Azocasein | 14.3 ± 2.5 | 207.5 ± 7.2 | 31.5 ± 2.61 | 397.6 ± 14.4 |
BSA | 4.3 ± 0.3 | 30.7 ± 2.9 | 6.1 ± 0.9 | 82.2 ± 15.3 |
Suc-AAPF-pNa | 600.1 ± 20.0 | 2003.4 ± 108.7 | 763.9 ± 38.5 | 2411.8 ± 83.0 |
The values are means ± standard deviations of three independent experiments.