TABLE 2.

Kinetic parameters of the WT protease and PBL5X^a

Temperature	Km (mM)		kcat (s−1)
	WT protease	PBL5X	WT protease	PBL5X
25°C	0.3 ± 0.03b	0.45 ± 0.06	51 ± 5b	113 ± 10
60°C	0.61 ± 0.04b	0.88 ± 0.07	348 ± 27b	606 ± 69
80°C	0.95 ± 0.07	1.77 ± 0.27	508 ± 16	975 ± 91

^aThe kinetic parameters were determined at different temperatures using suc-AAPF-pNA as the substrate. The values are means ± standard deviations of three independent experiments.

^bData obtained from Yang et al. (27).