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Engineered pathway for the biosynthesis of colored anthocyanin, cyanidin 3-O-glucoside (C3G), in E. coli. (A) Mechanism catalyzing the formation of cyanidin from (+)-catechin by anthocyanidin synthase (ANS) and the transfer of a glucose group from UDP-glucose to cyanidin to yield C3G by 3-O-glycosyltransferase (3GT). (B) C3G-producing strains can be efficiently screened by using a colorimetric assay.
