TABLE 1.
Primers used in this study
| Oligonucleotide | Primer sequence (5′–3′) | Function of underlined bases |
|---|---|---|
| CYP-sb21-F | GGAATTCCATATGGACACCGTTAATCTG | NdeI |
| CYP-sb21-R1 | CGGAATTCGCGCAGCAGAACCGGCAG | EcoRI |
| Fdx-F | GGAATTCCATATGATGGCAACCTACAAGG | NdeI |
| Fdx-R | CCGCTCGAGTTAGTAGAGGTCTTCTTC | XhoI |
| FdR-F | CGGGATCCATGTTGAATGCGAGTGTG | BamHI |
| FdR-R | CCCAAGCTTCTAGTAGGTTTCAACATGCC | HindIII |
| RhFRED-F | CAGATTCATATGGTGCTGCACCGCCATCAA | NdeI |
| RhFRED-R | CCCAAGCTTGAGTCGCAGGGCCAGCCG | HindIII |
| CYP-sb21-R2 | TTGATGCCGGTGCAGGCGCAGCAGAACC | cyp-sb21 overhang |
| RhFRED-Fdx-F | GGTTCTGCTGCGCCTGCACCGGCATCAAC | FMN domain overhang |
| RhFRED-Fdx-R | CCCAAGCTTTTATGCCGTCAGTTCTTC | HindIII |
| GDH-F | GGAATTCCATATGTATAAAGATTTAAAGG | NdeI |
| GDH-R | CGCGGATCCTTATCCGCGTCCTGCTTG | BamHI |
| CYP-sb21-Duet-F | GGAATTCCATATGGACACCGTTAATCTG | NdeI |
| CYP-sb21-Duet-R | CCGCTCGAGTCAGCGCAGCAGAACCGGCAG | XhoI |