FIG 6.

Flocculation and FLO gene copy number in S. pastorianus A1, A1+B11, and A2. (Top) The copy number of the annotated FLO genes was predicted using Magnolya (28). The copy number of the contig harboring the FLO gene was compared to the copy number of the contigs comprising the rest of the chromosome. *, the gene copy number matches the chromosome value. The sequence analysis of the FLO1 and FLO9 genes did not enable an unequivocal assignment of an S. cerevisiae or S. eubayanus allele type; these genes are therefore labeled “unknown.” (Bottom left) Flocculation properties of the A1, A1+B11, and A2 strains. The strains were grown in WMM medium at 20°C for 38 h. Cells were sampled by centrifugation, and flocculence was analyzed with the modified Helm's test (38, 39). The flocculation coefficient was calculated from the blank-corrected average from six replicates. (Bottom right) Correlation between the flocculation coefficient and the copy number of LgFLO1.