FIG 3.

(A to C) qPCR analysis of expression levels of terpene biosynthetic genes in the three selected tri4-overexpressing transformants relative to the levels of expression of these genes in the control strain, T34-5.27-b1, in mycelia collected from 96-h cultures. Comparisons and graphic representations were carried out using REST software (36). Values indicated with asterisks (boxed in the graphic representation) correspond to genes significantly differentially expressed (P ≤ 0.05) in comparison with the reference condition. Strain Ta37 was used to compare the levels of expression of tri4 in the T34-5.27-tri4 transformants, since tri4 is not present in strain T34.