TABLE 2.
Oligonucleotide primers used in this study
| Primer | Sequence (5′-3′)a | Restriction site |
|---|---|---|
| efUpF | AAACTCGAGTCATTATTAATGATAAAACGACGAT | XhoI |
| efUpR | TTTTCTTATTTCCCATCTAGGATAA | |
| efDownF | TTATCCTAGATGGGAAATAAGAAAAACTCCTCGTTTTGCTTGGG | |
| efDownR | AAAAGATCTTAATTTCACGGTTAGCCCCTG | BglII |
| tUpF | AGGCTCGAGAAAAACGGCTGCGGCATCTATG | XhoI |
| tUpR | TCTGTCGACGCTGCTTCACCGTCACAAAGGC | SalI |
| tDownF | GATGTCGACAAAGGAAATCTCATGGGAATTC | SalI |
| tDownR | CCTCTGCAGTTAACCAGTGTATGAACCGTAG | PstI |
| KanF | GTGTAGGCTGGAGCTGCTTC | |
| KanR | ATGGGAATTAGCCATGGTCC | |
| EupF | CTGGATACCACCTGTTATATGGTG | |
| EupR | GAAGCAGCTCCAGCCTACACAGATACCTTCTTGACATATAACCGG | |
| EdownF | GGACCATGGCTAATTCCCATCGTGGCGAGCAGGACGC | |
| EdownR | GAAACCAATGAAGAGATTATTGAAG | |
| AupF | GCGCCAGGGCAAGTATTTAC | |
| AupR | GAAGCAGCTCCAGCCTACACACATGTTCCTCTGTGGTAGATGATG | |
| DdownF | GGACCATGGCTAATTCCCATAGTTTGCGGAACTCGGTGTT | |
| DdownR | GATTCATACCACGACTATCAACGC | |
| efF | AACATATGACTGATAAAAAATTTTTAGACCAA | NdeI |
| efR | ATAGTCGACTTAGTGGTGGTGGTGGTGGTGTTCAAAGAGTGCTTGACCAAGG | SalI |
| SPF | ATGACTGATAAAAAATTTTTAGACC | |
| SPR | AGCAGATGCACCAGAAAGAC | |
| PtatF | AAGGATCCGTGAAAAGACAAATCGTGGACTC | BamHI |
| PtatR | GGTCTAAAAATTTTTTATCAGTCATGAATGTCTCCTATTTTCAAGATAGT | |
| PcmF | AAAAGATCTCTGCAGTCGACGGCAATAGTTAC | BglII |
| PcmR | GGTCTAAAAATTTTTTATCAGTCATTTGATATGCCTCCTAAATTTTTATC | |
| cmF | GTCTTTCTGGTGCATCTGCTATGAACTTTAATAAAATTGATTTAG | |
| cmR | AAAAGATCTCTGTAATATAAAAACCTTC | BglII |
| RepF | AAAAGATCTTCAGCAAATAACTCTTTTTCTTTG | BglII |
| RepR | AAAAGATCTATTGTAAAAGTGTCACTGCTGCTAG | BglII |
| P1F | AAAAGATCTAGCGCGCCAAAGAAGTAG | BglII |
| P1R | AAGAATTCATATGTCGAGCCTCCTGAAGTACTG | NdeI |
| TatCF | AACATATGGCAAGAAGTAGAGATGAGATG | NdeI |
| TatAR | AAGTCGACCTAGTCTTTAGTTTTGTCTTCTTCG | SalI |
| RT-16sF | CTAACTACGTGCCAGCAGC | |
| RT-16sR | GGTTGAGCCACAGCCTTTA | |
| RT-efF | ATGATGAACAAGTAGCCTTCC | |
| RT-efR | CCTTACGGTCACCAATAGC | |
| RT-tatF | GCTGTTCTCTTTGAATTTCCTGTAG | |
| RT-tatR | GTGTCAGAATCACCGCCAAA | |
| ST tufF | GGTGCGATCCTTGTAGTTGCA | |
| ST tufR | ACACCAACCTGACGTGAAAGAA | |
| RPOST-F | ACTGTCATTGTTGCTTGGAATG | |
| RPOST-R | AGCTGAGGTTACTGCTGGAGAT |
a
The restriction sites in the primer sequences are underlined.