FIG 5.

Efficiency of silencing of AfPXG at the gene transcript and enzymatic activity levels. (A) Two siRNAs specific for PXG, siRNAPXG1 and siRNAPXG2 (Table 1), were delivered to the protoplast via the Lipofectin reagent at various concentrations. Treatment of the protoplasts without (−) siRNA or with nonspecific control siRNA, (+) siRNACt, used as a negative control, was performed. AfPXG transcripts were quantified by qRT-PCR. (B) AfPXG activities were measured by sulfoxidation of thiobenzamide (light gray), hydroxylation of aniline (dark gray), and epoxidation of oleic acid (black). Values are means ± SDs (n = 3). The activities of lines in which AfPXG was silenced differed significantly from those of the control when analyzed by the t test (*, P < 0.05; **, P < 0.01).