FIG 3.

Effect of calcium on C- and E-genotype strain attachment to chitin. The chitin attachment efficiencies of two C-genotype strains (C7184 and MO6-24) and two E-genotype strains (JY1305 and JY1701) incubated in 1/2-strength ASW either with or without 0.018% (wt/vol) Ca²⁺ are shown. Error bars represent the standard errors of the means from three biological replicates for each strain and condition. Calcium enhances the efficiency of attachment of E-genotype strains to chitin but has no effect on the efficiency of C-genotype strain attachment. Different letters indicate statistically significant differences (one-way ANOVA, P < 0.0001).