FIG 1.
Transcriptional analysis of the AGDI operon of L. lactis subsp. cremoris CECT8666. (A) Genetic organization of the AGDI cluster and surrounding regions. The putative aguR promoter (PaguR), the aguB promoter (PaguB), and the termination regions (
) are indicated. (B) RT-PCR amplification of intergenic regions was conducted using total RNA extracted from cells grown in the presence of 20 mM agmatine. Five set of primers were designed to amplify the intergenic regions: ycaC-aguR (primer pair 1, lanes 1), aguR-aguB (primer pair 2, lanes 2), aguB-aguD (primer pair 3, lanes 3), aguD-aguA (primer pair 4, lanes 4), and aguA-aguC (primer pair 5, lanes 5). Negative controls were run with the same RNA samples but without reverse transcriptase. Positive controls were run with chromosomal DNA. Lanes M, DNA molecular size markers.