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Production of putrescine by L. lactis subsp. cremoris CECT8666 (WT) and the ΔaguR deletion mutant at different agmatine concentrations. Both strains were grown in GM17 medium supplemented with 0, 0.05, 0.1, 0.25, 0.5, 1, 5, 10, or 20 mM agmatine for 24 h. Supernatants were analyzed by UHPLC to determine the putrescine concentration in the extracellular medium.
