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Cloning and assay of PaguR and PaguB activity, reported as GFP fluorescence, in the presence and absence of 20 mM agmatine. The genetic fusions PaguR-gfp, PaguB-gfp, and PaguR-aguR-PaguB-gfp were transformed into L. lactis subsp. cremoris NZ9000 cells, and promoter activity was determined by measuring whole-cell fluorescence (250 μl of cells) at a similar OD600. The values shown are the means from three replicates; standard deviations are indicated by bars. a.u., arbitrary units.
