FIG 5.
Effect of the agmatine concentration on the transcriptional activity of the AGDI cluster promoters, measured by whole-cell fluorescence. L. lactis subsp. cremoris NZ9000 cells harboring either the PaguR-gfp (A), the PaguB-gfp (B), or the PaguR-aguR-PaguB-gfp (C) genetic fusion were grown in GM17 medium supplemented with 0, 10−6, 10−5, 10−4, 10−3, 10−2, 0.1, 0.5, 1, 2, 5, 10, or 20 mM agmatine for 7 h, after which GFP fluorescence was monitored. The values shown are the means from three replicates; standard deviations are indicated by bars. a.u.: arbitrary units.