TABLE 2.
Primers used
| Primer | Functiona | Nucleotide sequence (5′ to 3′) | Source or reference |
|---|---|---|---|
| AguF | Intergenic region ycaC-aguR (F) | CGAACAGACAGCGTCCCTGA | This work |
| AgurNco | Intergenic region ycaC-aguR (R) | CCCCATGGGATTAGACCTACTTATCATATTATCA | This work |
| PtcBglII | Intergenic region aguR-aguB (F) | CCCCAGATCTAAGCATATGAAAAATCAGAACTTAG | This work |
| PTC3C | Intergenic region aguR-aguB (R) | CTTTAGTGTAATCTTCTGTTGTGATG | This work |
| RT1 | Intergenic region aguB-aguD (F) | CCTTATGATTTGAAAGCACAAG | This work |
| RT2 | Intergenic region aguB-aguD (R) | GAAAGAATAGCACTAAATAGAC | This work |
| RT3 | Intergenic region aguD-aguA (F) | TTATTGGAGAACTGATTATTAAAG | This work |
| AgDI3Cre | Intergenic region aguD-aguA (R) | CTTGAGCTTCAAATTCACCGGGC | This work |
| RT4 | Intergenic region aguA-aguC (F) | GGCGGTGGAAATATTCACTG | This work |
| RT5 | Intergenic region aguA-aguC (R) | TTGTGCTGTAGGGTCACTGC | This work |
| qAguR-F | aguR expression analysis (F) | CTATCGACAGGTTAAGCAAAGCAGTT | 13 |
| qAguR-R | aguR expression analysis (R) | TCCAAAGATGATGGCCATTATGC | 13 |
| qPTC-F | aguB expression analysis (F) | ACTTGGTGGACATGAAACAATAGAAGAT | 13 |
| qPTC-R | aguB expression analysis (R) | GTCAACACGTGCCATTATGATATCG | 13 |
| rpoA-F | rpoA reference gene (F) | CACGGGCAGGTTCAACTTG | 22 |
| rpoA-R | rpoA reference gene (R) | TTCCGGCTGACGAAAATAAAG | 22 |
| qtufF | EF-Tu reference gene (F) | TCTTCATCATCAACAAGGTCTGCTT | 13 |
| qtufR | EF-Tu reference gene (R) | GAACACATCTTGCTTTCACGTCAA | 13 |
| AguRBglII | Cloning of PaguR (F) | CCCCCCAGATCTGACAAGTTTGGCTCAGATTGCTTG | This work |
| PtcNco | Cloning of PaguB (R) | CCCCCCATGGTGTTTATTCCTCCTGAATAAAATAG | This work |
| AgurlicF | Cloning of aguR (F) | ATGGGTGGTGGATTTGCTATGTTAAATTATATTTATACTACTTTTT | This work |
| AgurlicR | Cloning of aguR (R) | TTGGAAGTATAAATTTTCTTGACTAAGTTCTGATTTTTCATATG | This work |
| Gffor | Cloning of gfp (F) | GGCCATGGGTGGTGGATTTGCTCAATTC | This work |
| Gfrev | Cloning of gfp (R) | CCGCATGCCTGCATTAATGATGGTG | This work |
| KO1F | Generation of pAGDIΔ1 (F) | CACACGAATTCGAAAAAAGCACTAAACCCTCC | This work |
| KO1R | Generation of pAGDIΔ1 (R) | CACACGAATTCTCCATTCAAAAAATGGAGCT | This work |
| KO2F | Generation of pAGDIΔ2 (F) | CACACGAATTCCTCAACCCCTTGGTAGCAAAGG | This work |
| KO2R | Generation of pAGDIΔ2 (R) | CACACGAATTCAAACGCTTTCTTTTTATAAATAAA | This work |
| KO3F | Generation of pAGDIΔ3 (F) | CACACGAATTCTTGCTTTTTAAAAAGATTAAATCCT | This work |
| KO3R | Generation of pAGDIΔ3 (R) | CACACGAATTCGTCAAAAGGTTTAGGAGGGTTTAG | This work |
| KO4F | Generation of pAGDIΔ4 (F) | CACACGAATTCAGTTGATTGTTTTAAGAAATCAACC | This work |
| KO4R | Generation of pAGDIΔ4 (R) | CACACGAATTCAAAACCCTTTGCTACCAAGGG | This work |
| KO-214AguR-AF2 | Generation of pIPLA1269 (F) | CACATGACTAGTTTAGAACCTAGAAACCCAGAAAC | This work |
| KO-214AguR-AR | Generation of pIPLA1269 (R) | AACTGCAGATTTAACATCATCGGATTAGACCTAC | This work |
| KO-214AguR-BF | Generation of pIPLA1713 (F) | AACTGCAGTCAGAACTTAGTCAATAATTTAAAAG | This work |
| KO-214AguR-BR | Generation of pIPLA1713 (R) | CCATCGATAACCGCATCAACAACTTC | This work |
a
F, forward; R, reverse; PaguR, aguR promoter; PaguB, aguB promoter.