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. 2015 Jul 10;4(8):1002–1015. doi: 10.1242/bio.012294

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Molecular analysis of novel salivary transcript encoding kunitz like protease inhibitor. (A) Web based identification of a salivary transcript encoding bi-functional thrombospondin (TSP) and Kunitz/BPTI domains, previously remains un-described from any mosquito species. The multiple sequence alignment (B) and phylogenetic (C) analysis of the selected Kunitz domain with identical features of a typical Kunitz/BPTI domain carrying cysteine (yellow color) pattern of CX(8)CX(15)CX(7)CX(12)CX(3)C. The predicted Kunitz sequence of ticks was considered as out group for the construction of N/J tree at 1000 boot strap values. (D) Up-regulation of salivary Kunitz (**P≤0.005) in response to blood feeding. Error bar represents standard deviation from three biological replicates.