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. 2015 Jul 17;4(8):1040–1051. doi: 10.1242/bio.012609

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Actin cytoskeleton in wildtype and mutant cdc8 cells expressing GFP-fimbrin, illustrating synthetic negative effect of cdc8^D16AK30A and fim1-GFP. All cells were fixed and stained with Alexa phallodin. Fim1-GFP fluorescence is weak following fixation. (A) cdc8^wt cells (SH30). (B) cdc8^D16AK30A cells (SH22). (C) cdc8^wt fim1-GFP cells (SH77). (D) cdc8^D16AK30A fim1-GFP cells (SH112). Note the absence of cables. (E) cdc8^E131A fim1-GFP cells (SH74). (F) cdc8^E138A fim1-GFP cells (SH75-A). The actin cytoskeleton appears normal in E and F.