Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 17;4(8):1040–1051. doi: 10.1242/bio.012609

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 6. — Comparison of the actin cytoskeleton using Alexa-phalloidin and LifeAct. (A) cdc8^wt cells (SH30) fixed and stained with Alexa-phalloidin showing long, typically straight actin cables parallel to the long axis of the cells. (B) cdc8^wt-LifeAct-GFP cells (KV587, ura⁻) (live cells confocal image) showing wavy cables with variable orientations in relation to the long axis of the cell. The contractile rings are similar in A and B. (C) cdc8^wt-LifeAct-GFP cells (KV587), fixed and stained with rhodamine-phalloidin (left, red channel), green channel showing GFP (center), and superimposed (right). The rhodamine and GFP images superimpose, showing that the phalloidin binds to the same structures that are labeled with GFP.