Figure 4.

Rfx6 Regulates a Subset of Genes Controlling β Cell Function and Directly Targets Gck and Abcc8

(A–J) qRT-PCR experiments for Gck, Kcnj11, Abcc8, Slc2a2, Pcsk1, Pax6, MafA, Nkx6.1, Pdx1, and Ucn3 on islets purified from controls and Rfx6^ΔBeta adult mice (9–11 weeks old), 5 days after tamoxifen injections. Grey triangles indicate the days of tamoxifen injections.

(K and L) ChIP-seq (anti-HA) data showing Rfx6 binding peaks in Abcc8 and Gck genes in 3HA-Rfx6 transfected Min6B1 cells.

(M and N) Quantitative ChIP (anti-Rfx6 antibody) in Min6B1 cells illustrating the binding of Rfx6 to X-boxes indicated in (K) and (L). PI and Rfx6 stand for preimmune and anti-Rfx6 serum.

Data are presented as mean ± SD on n = 4–5 samples; ^∗∗p < 0.01, ^∗p < 0.05.