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. 2015 Aug 20;5:13346. doi: 10.1038/srep13346

Figure 1. Induced haploid androgenesis by cold-shock in zebrafish (Danio rerio).

Figure 1

(a) Cleavage rate, survival rate at 24 h, and haploid rate of just-fertilized eggs cold-shocked at 1 ± 0.5 °C, 4 ± 0.5 °C, 7 ± 0.5 °C, and 10 ± 0.5 °C for 30 min. Different letters above the columns denote significant differences as determined by one-way ANOVA and Duncan’s multiple comparisons (P < 0.05). (b) Cleavage rate, survival rate at 24 h, and haploid rate of just-fertilized eggs cold-shocked at 7 ± 0.5 °C for 20, 30, 40, 50, or 60 min. Different letters above the columns denote significant differences as determined by one-way ANOVA and Duncan’s multiple comparisons (P < 0.05). (c–j) Relative DNA content and metaphase chromosomes of different ploidy larvae hatched from intact control eggs and eggs subjected to cold shock at 7 ± 0.5 °C for 30 min. (c) 1N abnormal larvae from the cold-shock group; (d) 1.3N abnormal larvae from the cold-shock group; (e) 2N normal larvae from the intact control group; (f) 3N abnormal larvae from the cold-shock group; (g) 1N abnormal larvae with 25 chromosomes from the cold-shock group; (h) 1.3N abnormal larvae with 32 chromosomes from the cold-shock group; (i) 2N normal larvae with 50 chromosomes from the intact control group; (j) 3N abnormal larvae with 75 chromosomes from the cold-shock group. Scale bars denote 10 μm.