FIG 5.

Depletion of gD- and gB-specific Abs from human serum sample Z7. To deplete human serum of glycoprotein-specific Abs, serum was incubated with biotinylated gD2 or gB2 that was bound to streptavidin-coated magnetic beads. (A) After depletion, the sample supernatant was tested by ELISA for binding to soluble gD2 (○) or gB2 (△); the optical density at 405 nm (OD₄₀₅) is shown on the y axis, and the serum dilution is indicated on the x axis of each ELISA graph. Next, the depleted sample was tested for the ability to neutralize either HSV-2 (B) or HSV-1 (C). Plaque numbers were determined for each sample and plotted as a percentage of plaques obtained in the absence of human serum (y axis). Serum dilutions are indicated on the x axis. Curves where the neutralization activity was successfully depleted are highlighted with a gray background.