FIG 4.
Analysis of environmentally exposed animal terminal disease state. IHC results and representative RT-QuIC data for deer E-1 and E-2 are displayed. Results are for one replicate each for serial dilutions of obex (10−3 to 10−6), retropharyngeal lymph node (10−2 to 10−4), and tonsil (10−2 to 10−4) samples. Each serial dilution was repeated in two experiments, with four replicates in each experiment. The number of total positive replicates for each dilution is noted. IHC staining for PrPCWD is characterized by red granular deposits in the neuropil of the obex and in germinal centers of lymphoid follicles in the tonsil and retropharyngeal lymph node. IHC staining was performed with the antibody F99/97.6.1. Magnification, ×20.