FIG 1.
SIVsmm and SIVmac efficiently use sooty mangabey but not rhesus macaque CXCR6 in vitro. Target 293T cells were transfected with smCD4 plus smCCR5, smCXCR6, or smGPR15 plasmid DNA (A) or rmCD4 plus rmCCR5, rmCXCR6, or rmGPR15 plasmid DNA (B) and then infected with luciferase-expressing pseudotyped virions carrying the indicated Envs. Cells transfected with CD4 alone (“none”) served as negative controls. Infection was measured by luciferase activity 3 days postinfection, and data were normalized to 100% entry in target cells expressing species-matched CD4 plus CCR5 (n = 3, means ± SDs).