FIG 3.
SIV infection of rhesus macaque PBMCs is highly CCR5 dependent, whereas sooty mangabey PBMC infection is partially CCR5 independent. Primary PBMCs from sooty mangabeys (A) and rhesus macaques (B) were stimulated with ConA and IL-2 for 3 days, treated or not for 1 h with maraviroc (10 μM), and then infected with replication-competent SIVsmm D215, SIVsmm M935, SIVsmm E660, SIVmac 239, or SIVmac 251-RZu4. Culture supernatants were collected periodically postinfection. Data represent peak supernatant p27 Gag production and are shown as the percentage of entry in maraviroc-treated PBMCs relative to that in vehicle-treated PBMCs. Each symbol represents cells from a unique sooty mangabey or rhesus macaque. SIVsmm M935 failed to replicate in rmPBMCs and is not shown in panel B.