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. 2015 Aug 19;89(18):9581–9590. doi: 10.1128/JVI.01082-15

FIG 3.

FIG 3

Detection of FMDV-specific antibody-secreting cells in lymphoid organs of the respiratory tract from vaccinated and further infected cattle. Prescapular (PSL) and mandibular lymph nodes (ML), pharyngeal tonsils (PhT), lateral (LRL) and medial retropharyngeal lymph nodes (MRL), tracheobronchial lymph nodes (TBL), and spleen (S) from FMD-vaccinated bovines were processed and assayed by the FMDV-ASC ELISPOT assay at 7 and 29 dpv and between 2 and 6 days postoronasal infection. Results are expressed as the number of FMDV-specific ASC per 5 × 105 extracted MNC, and each bar represents the average value for 2 individuals ± standard errors of the means. Significant differences in mean ASC numbers/5 × 105 total MNC were the following: a, PSL > ML, MRL, LRL, PhT, TBL, and S (P < 0.05) at 29 dpv; b, PSL > ML, MRL, LRL, PhT, TBL, and S (P < 0.01) at 2 dpi; c, TBL > PSL, ML, LRL, and PhT (P < 0.001) at 5 dpi; d, TBL > S (P < 0.01) at 5 dpi; e, MRL > PSL, ML, LRL, and PhT (P < 0.05) at 6 dpi; and f, TBL > PSL, ML, MRL, LRL, PhT, and S (P < 0.001) at 6 dpi.